Immobilization of stable thylakoid vesicles in conductive nanofibers by electrospinning.

نویسندگان

  • Nicholas M Bedford
  • G Douglas Winget
  • Srikoundinya Punnamaraju
  • Andrew J Steckl
چکیده

Electrospun fibers consisting of poly(3,4-ethylenedioxythiophene)/poly(styrene sulfonate) (PEDOT/PSS) and poly(ethylene oxide) (PEO) have been used to successfully encapsulate and stabilize thylakoid membrane vesicles isolated from spinach. Light-driven electronic properties were measured. Fibers with immobilized thylakoids show higher electrical conductivity compared with fibers without thylakoids under white light conditions. This is attributed to the electron-generating photosynthetic reactions from the thylakoids. Electron and optical microscopy show the presence of thylakoid vesicles within the fibers using lipid-specific stains. After electrospinning into fibers, the thylakoid vesicles still exhibit an ability to produce a light-driven electron gradient, indicating that activity is preserved during the electrospinning process. These electrospun fibers provide an excellent example of incorporating photosynthetic function into an artificial system.

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عنوان ژورنال:
  • Biomacromolecules

دوره 12 3  شماره 

صفحات  -

تاریخ انتشار 2011